new insight into the immunomodulatory mechanisms of tretinoin in nmri mice
Authors
abstract
objective(s):recent evidence have proposed that tretinoin produced in the gut preferentially promote differentiation of foxp3+treg cells but inhibits th17 lymphocytes, and this may be the main immunomdulatory mechanism of tretinoin in vivo. this study was done to investigate the effects of tretinoin in outbred white mice after challenge with sheep red blood cells (srbc). materials and methods: twenty male nmri-mice randomly allocated in two equal groups. mice were treated with 1×109 srbcs emulsified in cfa intraperitoneally twice with one weak interval. animals were bled 5 days after last injection. moreover, 48 hr before bleeding time, 1×109 srbcs were injected into the left hind foot pad of mice. tretinoin (25 mg/kg-every other day) were intraperitoneally injected into the treatment group from the beginning of the study and continued throughout the study. the levels of anti-srbc antibody and the specific cellular immune responses were measured by microhemagglutination test and footpad thickness, respectively. moreover, splenocytes were checked for proliferation rate, respiratory burst, cytokine production and foxp3+treg cells frequency. results: tretinoin markedly alleviated cellular immunity and concurrently potentiated humoral immunity after mice challenge with srbcs. furthermore, aside from reducing nbt reduction and lymphocyte proliferation, tretinoin markedly suppressed the secretion of interleukin-17 and conversely, increased the production of interleukin-10. however, the level of ifn-γ and the frequency of foxp3+treg cells did not alter significantly. conclusion: the in vivo immunomudlatoty effects of tretinoin may be partly due to immune deviation from pro-inflammatory cytokine interleukin-17 to anti-inflammatory cytokine interleukin-10, but not absolutely depend on the expansion of foxp3+treg cells.
similar resources
New insight into the immunomodulatory mechanisms of Tretinoin in NMRI mice
Objective(s):Recent evidence have proposed that Tretinoin produced in the gut preferentially promote differentiation of FoxP3+Treg cells but inhibits Th17 lymphocytes, and this may be the main immunomdulatory mechanism of Tretinoin in vivo. This study was done to investigate the effects of Tretinoin in outbred white mice after challenge with sheep red blood cells (SRBC). Materials and Methods:...
full textNew insight into the immunomodulatory mechanisms of Tretinoin in NMRI mice
OBJECTIVES Recent evidence have proposed that Tretinoin produced in the gut preferentially promote differentiation of FoxP3+Treg cells but inhibits Th17 lymphocytes, and this may be the main immunomdulatory mechanism of Tretinoin in vivo. This study was done to investigate the effects of Tretinoin in outbred white mice after challenge with sheep red blood cells (SRBC). MATERIALS AND METHODS T...
full textNew Insight into the Relation between Adiponectin and Diabetes
Diabetes mellitus is a group of metabolic diseases. Insulin resistance is defined as a state that needs more insulin to get the biological effects achieved by a lower amount of insulin in the normal state. Several molecules which are secreted through adipocytes have been involved in insulin resistance and type 2 diabetes mellitus. Adiponectin as a 244 amino acid protein is exclusively expressed...
full textThe Immunomodulatory Effects and Mechanisms of Tim-3 Action in the Early Stage of Mice with Severe Acute Pancreatitis
Background: Tim-3 has been considered as an ideal target for the immunotherapy of inflammation, but it is unclear whether Tim-3 also plays an important role in acute pancreatitis (AP), as well. Objective: To identify the immunomodulatory effects and mechanisms of Tim-3 action in the early stages of severe acute pancreatitis in mice. Methods: Ma...
full textMy Resources
Save resource for easier access later
Journal title:
iranian journal of basic medical sciencesجلد ۱۷، شماره ۹، صفحات ۶۳۲-۶۳۷
Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023